cd55  (Cell Signaling Technology Inc)

Journal: World Journal of Surgical Oncology

Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

doi: 10.1186/s12957-025-04051-2

Figure Lengend Snippet: Expression of CD55 mRNA and protein in normal thymus, thymoma, and TSCC. The expression levels of CD55 mRNA ( A ) and protein ( B ) were significantly elevated progressively in normal thymus tissue, thymoma, and TSCC

Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

doi: 10.1186/s12957-025-04051-2

Figure Lengend Snippet: Intensities of CD55 expression in TETs (IHC) scored 0: negative expression ( A , 40×; a, 200×); scored 1: low expression ( B , 40×; b, 200×); scored 2: moderate expression ( C , 40×; c, 200×); scored 3: high expression ( D , 40×; d, 200×)

Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

doi: 10.1186/s12957-025-04051-2

Figure Lengend Snippet: Proportion of CD55 expression in TETs (IHC) scored 0: negative expression ( A , 40×; a, 200×); scored 1: 1% − 30% positive cells ( B , 40×; b, 200×); scored 2: 30% − 60% positive cells ( C , 40×; c, 200×); scored 3: ≥ 60% positive cells ( D , 40×; d, 200×)

Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

doi: 10.1186/s12957-025-04051-2

Figure Lengend Snippet: Kaplan-Meier Survival Analysis in TETs The results demonstrated that PFS of TETs was closely related to CD55 mRNA ( A , p < 0.0001), CD55 protein ( B , p < 0.0001), tumor completeness of resection ( C , p < 0.0001), histological type ( D , p < 0.0001), Masaoka-Koga stage ( E , p < 0.0001), and adjuvant therapy ( F , p = 0.0009), but no with gender ( G , p = 0.1805) or MG ( H , p = 0.7269)

Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

Techniques: Adjuvant

Journal: World Journal of Surgical Oncology

Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

doi: 10.1186/s12957-025-04051-2

Figure Lengend Snippet: The relationship between CD55 and the clinical pathological features of patients The expression levels of CD55 mRNA and protein were higher in R1/R2 group ( A ), higher histological grade ( B ), Masaoka-Koga stage III/IV ( C ), adjuvant therapy ( D ) compared with the corresponding R0 group, normal thymus tissues, and stage I/II, and no-adjuvant therapy. Moreover, the expression of CD55 protein was significantly higher expressed in ≥ 60 years compared with ˂60 years ( E ). Additionally, CD55 protein expression was higher in males than that in females ( F ), whereas CD55 mRNA did not show similar results ( E, F ). Patients without MG showed higher CD55 mRNA and protein expression levels than those with MG, however, this difference was not statistically significant ( G ). There was also no statistically significant relationship between tumor diameter and CD55 expression ( H ). When CD55 protein was highly expressed, the mRNA level was also high ( I ).

Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

Techniques: Expressing, Adjuvant

Journal: World Journal of Surgical Oncology

Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

doi: 10.1186/s12957-025-04051-2

Figure Lengend Snippet: Kaplan-Meier Survival Analysis in high-risk TETs The results demonstrated that PFS of high-risk TETs was closely related to high expression of CD55 mRNA (A, p < 0.0001) and CD55 protein (B, p = 0.0168)

Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

Techniques: Expressing

Journal: Cell Reports Medicine

Article Title: RSAD2 : A pathogenic interferon-stimulated gene at the maternal-fetal interface of patients with systemic lupus erythematosus

doi: 10.1016/j.xcrm.2025.101974

Figure Lengend Snippet: RSAD2 is highly expressed in pregnant patients with SLE (A) Heatmap showing the expression of interferon-stimulated genes (ISGs) in the peripheral blood mononuclear cells (PBMCs) of healthy women (Healthy PBMCs), female patients with SLE (SLE PBMCs), healthy pregnant women (Healthy pregnancy PBMCs), and pregnant patients with SLE (SLE pregnancy PBMCs). (B) Heatmap showing the expression of ISGs in the decidua and villi of healthy early pregnant women and pregnant patients with SLE. (C) Heatmap showing the expression of ISGs in the decidual and trophoblast organoids from the first trimester decidual gland or trophoblast cells (passage 1, day 5), treated with 1,000 U/mL IFN-α or PBS for 12 h. (A, B, and C). The 2 −ΔCt values of ISGs were calculated from the ISG qPCR array data. (D) Representative IHC image of IFNAR1 and CD55 (expressed in DSCs) co-staining of decidual tissue sections (top), and representative IHC image of IFNAR1 and EPCAM (expressed in VCTs, the proliferative villous cytotrophoblast cells) co-staining of chorionic villi sections (bottom); scale bar, 50 μm. (E) Representative IHC image of decidual organoids (top) from the first trimester decidual glands (passage 1, day 5) and of trophoblast organoids (bottom) from the first trimester trophoblast cells (passage 1, day 5); scale bar, 50 μm. (F) Expression of ISGs in decidual CD56 + NK cells, CD3 + T cells, CD14 + macrophages, decidual stromal cells (DSCs), and trophoblasts ( n = 3); the 2ˆ −ΔΔCt values of ISGs were calculated from the qPCR data. (G and H) Western blot showing RSAD2 and ISG15 expression in DSCs and trophoblasts, treated with 1,000 U/mL IFN-α or PBS for 12 h. Data were analyzed using the unpaired t test and are presented as the mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns , not significant.

Article Snippet: Slices were stained using antibodies against: IFNAR1 (rabbit, Invitrogen, 1:100, PA5-120652), CD55 (rabbit, CST, 1:1200, 31759), EPCAM (rabbit, 1:200, CST, 93790), COX-2 (rabbit, 1:500, CST, 12282), F4/80 (rabbit, 1:200, CST, 70076), and CD31 (rabbit, 1:200, CST, 77699) overnight at 4°C.

Techniques: Expressing, Staining, Western Blot

Journal: Cell Reports Medicine

Article Title: RSAD2 : A pathogenic interferon-stimulated gene at the maternal-fetal interface of patients with systemic lupus erythematosus

doi: 10.1016/j.xcrm.2025.101974

Figure Lengend Snippet:

Article Snippet: Slices were stained using antibodies against: IFNAR1 (rabbit, Invitrogen, 1:100, PA5-120652), CD55 (rabbit, CST, 1:1200, 31759), EPCAM (rabbit, 1:200, CST, 93790), COX-2 (rabbit, 1:500, CST, 12282), F4/80 (rabbit, 1:200, CST, 70076), and CD31 (rabbit, 1:200, CST, 77699) overnight at 4°C.

Techniques: Recombinant, SYBR Green Assay, Multiplex Assay, Immunohistochemical staining, Staining, Reverse Transcription, Modification, Plasmid Preparation, Software, Microscopy

Journal: Cell Reports Medicine

Article Title: RSAD2 : A pathogenic interferon-stimulated gene at the maternal-fetal interface of patients with systemic lupus erythematosus

doi: 10.1016/j.xcrm.2025.101974

Figure Lengend Snippet: RSAD2 is highly expressed in pregnant patients with SLE (A) Heatmap showing the expression of interferon-stimulated genes (ISGs) in the peripheral blood mononuclear cells (PBMCs) of healthy women (Healthy PBMCs), female patients with SLE (SLE PBMCs), healthy pregnant women (Healthy pregnancy PBMCs), and pregnant patients with SLE (SLE pregnancy PBMCs). (B) Heatmap showing the expression of ISGs in the decidua and villi of healthy early pregnant women and pregnant patients with SLE. (C) Heatmap showing the expression of ISGs in the decidual and trophoblast organoids from the first trimester decidual gland or trophoblast cells (passage 1, day 5), treated with 1,000 U/mL IFN-α or PBS for 12 h. (A, B, and C). The 2 −ΔCt values of ISGs were calculated from the ISG qPCR array data. (D) Representative IHC image of IFNAR1 and CD55 (expressed in DSCs) co-staining of decidual tissue sections (top), and representative IHC image of IFNAR1 and EPCAM (expressed in VCTs, the proliferative villous cytotrophoblast cells) co-staining of chorionic villi sections (bottom); scale bar, 50 μm. (E) Representative IHC image of decidual organoids (top) from the first trimester decidual glands (passage 1, day 5) and of trophoblast organoids (bottom) from the first trimester trophoblast cells (passage 1, day 5); scale bar, 50 μm. (F) Expression of ISGs in decidual CD56 + NK cells, CD3 + T cells, CD14 + macrophages, decidual stromal cells (DSCs), and trophoblasts ( n = 3); the 2ˆ −ΔΔCt values of ISGs were calculated from the qPCR data. (G and H) Western blot showing RSAD2 and ISG15 expression in DSCs and trophoblasts, treated with 1,000 U/mL IFN-α or PBS for 12 h. Data were analyzed using the unpaired t test and are presented as the mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns , not significant.

Article Snippet: Rabbit anti-CD55 antibody , CST , Cat# 31759; RRID: AB_2799010.

Techniques: Expressing, Staining, Western Blot

Journal: Cell Reports Medicine

Article Title: RSAD2 : A pathogenic interferon-stimulated gene at the maternal-fetal interface of patients with systemic lupus erythematosus

doi: 10.1016/j.xcrm.2025.101974

Figure Lengend Snippet:

Article Snippet: Rabbit anti-CD55 antibody , CST , Cat# 31759; RRID: AB_2799010.

Techniques: Recombinant, SYBR Green Assay, Multiplex Assay, Immunohistochemical staining, Staining, Reverse Transcription, Modification, Plasmid Preparation, Software, Microscopy